Journal: International Journal of Molecular Sciences
Article Title: Long Noncoding RNA VLDLR-AS1 Levels in Serum Correlate with Combat-Related Chronic Mild Traumatic Brain Injury and Depression Symptoms in US Veterans
doi: 10.3390/ijms25031473
Figure Lengend Snippet: Exosomes derived from CNS were isolated using the L1CAM antibody for pull-down during exosome purification. RNA was isolated from CNS-derived exosomes (brain) and from serum (total), and qPCR was performed using primers specific for lncRNAs MALAT1, GAS5, NEAT1 and VLDLR-ASI. Absolute quantities were determined by normalizing to U6. Statistical analysis was performed in GraphPad Prism SPSS Analysis Software V.10.0.2 to calculate the geo mean 95% CI in both total and brain samples for VLDLR-AS1, NEAT1, MALAT1 and GAS5 levels.
Article Snippet: Descriptive statistics for all demographic and clinical variables were calculated using GraphPad Prism SPSS Analysis Software V.10.0.2 (Graphpad Software, IBM SPSS Inc., Boston, MA, USA).
Techniques: Derivative Assay, Isolation, Purification, Software